abstract：:To determine whether chloroquine can specifically elute HLA antigens and beta 2-microglobulin (beta 2-M) from the platelet surface, quantitative immunofluorescence flow cytometry and monoclonal antibodies were used to show that HLA antigens and beta 2-M were proportionally eluted from the platelet surface without affe...

journal_title：Transfusion

authors： Kao KJ

更新日期：1988-01-01 00:00:00

abstract：:After immunization with native human red cells and red cells infected with Plasmodium falciparum, mouse splenocytes were fused with a myeloma cell line to obtain hybridomas. Among the antibodies specific for blood group antigens, four antibodies (iB3C4, F12, MR432, and iB4) directed against epitopes related to the Rh ...

journal_title：Transfusion

authors： Rouger P,Edelman L

更新日期：1988-01-01 00:00:00

abstract：:Fresh human blood was collected in citrate-phosphate-dextrose, frozen by a high-glycerol technique, and stored at -80 degrees C. The red cells were thawed, deglycerolized, and resuspended in a final wash solution, ADSOL (Fenwal Laboratories), or an additive solution (AS) containing glucose, adenine, mannitol, and phos...

journal_title：Transfusion

authors： Moore GL,Ledford ME,Mathewson PJ,Hankins DJ

更新日期：1987-11-01 00:00:00

abstract：:Standard packed red cell (PRC) units can be depleted of leukocytes and platelets if they are transfused through a blood administration set in which the usual 170-mu filter has been replaced by a leukocyte removal filter (Sepacell R-500). During a 6-month period, 1550 PRC units were transfused through this filter in 61...

journal_title：Transfusion

authors： Sirchia G,Rebulla P,Parravicini A,Carnelli V,Gianotti GA,Bertolini F

更新日期：1987-09-01 00:00:00

abstract：:Anti-Emm, an antibody to a public red cell antigen, was found in the serums of four Emm-propositi and in the serum of the Emm-brother of one of them. Three of the propositi were men, and their antibodies, as well as that of the compatible brother, were believed to be "naturally occurring." Since there are two Emm-sibl...

journal_title：Transfusion

authors： Daniels GL,Taliano V,Klein MT,McCreary J

更新日期：1987-07-01 00:00:00

abstract：:During the first 5 years (1981-1985) of the liver transplantation program in Pittsburgh, a total (preoperative, intraoperative, and postoperative) of 18,668 packed red cell units, 23,627 fresh-frozen plasma units, 20,590 platelet units, and 4241 cryoprecipitate units was transfused for the procedures. This represents ...

journal_title：Transfusion

authors： Lewis JH,Bontempo FA,Cornell F,Kiss JE,Larson P,Ragni MV,Rice EO,Spero JA,Starzl TE

更新日期：1987-05-01 00:00:00

abstract：:The serum of a pregnant woman whose red cells typed as Co(a-b-) contained an alloantibody that, at the time of the infants delivery, was hemolytic in vitro and had a titer of 32,000 by the antiglobulin test. This antibody, which reacted with cells of all Colton-phenotypes except the proposita's own cells, showed very ...

journal_title：Transfusion

authors： Lacey PA,Robinson J,Collins ML,Bailey DG,Evans CC,Moulds JJ,Daniels GL

更新日期：1987-05-01 00:00:00

abstract：:Prospective-concurrent audits and medical consultation were associated with a 56 percent decrease in the use of platelet concentrates at a tertiary care medical center. This study documents that mandatory pretransfusion review of component requests can alter hospital transfusion practices. ...

journal_title：Transfusion

authors： Simpson MB

更新日期：1987-03-01 00:00:00

abstract：:This study evaluated the effect of pneumatic tube transport on blood bank specimens and products. No important differences were found between aliquots transported in the tube system and those stored in the laboratory as controls. ABO, Rh, antibody detection or identification, direct antiglobulin testing, and elution w...

journal_title：Transfusion

authors： Tanley PC,Wallas CH,Abram MC,Richardson LD

更新日期：1987-03-01 00:00:00

abstract：:A prospective double-blind study compared a manual hexadimethrine bromide (Polybrene) antiglobulin antibody detection test (P-AHG) and crossmatch with the albumin-antiglobulin antibody detection test and saline-antiglobulin crossmatch routinely used in our laboratory. A total of 10,084 pretransfusion blood samples fro...

journal_title：Transfusion

authors： Mintz PD,Anderson G

更新日期：1987-03-01 00:00:00

abstract：:This study evaluated various algorithms for the generation of a red cell antibody identification panel by computer. The algorithms depended on schemes that optimized Fisher's exact p value for each specificity in the panel rather than using predetermined formulations of desired phenotypic combinations. Panels produced...

journal_title：Transfusion

authors： AuBuchon JP,Roberts GC

更新日期：1987-01-01 00:00:00

abstract：:A 20-year-old man with chronic granulomatous disease (CGD) and who was receiving granulocyte transfusions for a refractory liver abscess was studied to compare the kinetics of 111In-labeled granulocytes with those of two functional granulocyte assays, nitroblue tetrazolium reduction and chemiluminescence. Transfused g...

journal_title：Transfusion

authors： Elliott GR,Clay ME,Mills EL,Abramson JS,McCullough J,Quie PG

更新日期：1987-01-01 00:00:00

abstract：:A confidential self-administered questionnaire was given to all donors prior to blood donation (n = 95,917). The questionnaire describes acquired immunodeficiency syndrome (AIDS) high-risk groups and requires the donor to designate his blood for either laboratory purposes or for transfusion. Six-hundred and twenty-sev...

journal_title：Transfusion

authors： Nusbacher J,Chiavetta J,Naiman R,Buchner B,Scalia V,Herst R

更新日期：1986-11-01 00:00:00

abstract：:Many strains of Escherichia coli possess an antigen that reacts with naturally occurring antibodies to human red cell B antigens. The authors tested the hypothesis that the presence of naturally occurring anti-B isoagglutinins afford protection against the development of E. coli septicemia. The blood groups found in 1...

journal_title：Transfusion

authors： Wittels EG,Lichtman HC

更新日期：1986-11-01 00:00:00

abstract：:The effects of dextran therapy on pretransfusion compatibility testing were evaluated in 24 patients with a variety of vascular disorders. Specimens were drawn before, during, and after the administration of low-molecular-weight dextran (average molecular weight, 40 kd). Forty-three specimens were studied extensively ...

journal_title：Transfusion

authors： Bartholomew JR,Bell WR,Kickler T,Williams GM

更新日期：1986-09-01 00:00:00

abstract：:Platelet concentrates intended for transfusion to immunosuppressed patients are irradiated to minimize transfusion-induced graft-versus-host disease. Because few reports describe how irradiation influences stored platelets, the authors studied whether 5000 rad of gamma irradiation, the maximum dose currently used clin...

journal_title：Transfusion

authors： Moroff G,George VM,Siegl AM,Luban NL

更新日期：1986-09-01 00:00:00

abstract：:Mild-to-profound anemia, thrombocytopenia, and rarely neutropenia have been observed in patients with the acquired immune deficiency syndrome (AIDS). To investigate a possible immune mechanism, blood samples from 28 hospitalized AIDS patients, four asymptomatic homosexual men, four homosexual men with the AIDS-related...

journal_title：Transfusion

authors： McGinniss MH,Macher AM,Rook AH,Alter HJ

更新日期：1986-09-01 00:00:00

abstract：:A polyolefin plastic (PL 732) bag formulated without liquid plasticizer allows storage of platelets for 5 and, now, up to 7 days. In order to assess the leaching of compounds from this new plastic, extracts of the supernatant from platelet concentrates stored in these bags were analyzed by high-performance liquid chro...

journal_title：Transfusion

authors： Labow RS,Tocchi M,Rock G

更新日期：1986-07-01 00:00:00

abstract：:Seven hundred and sixty-three blood donors previously typed for HLA-A and B antigens were asked to consider donating bone marrow to unrelated patients for whom no HLA-matched sibling was available. Eighty-eight percent (668) indicated willingness to donate marrow, and of these, 615 (81%) participated in testing and 53...

journal_title：Transfusion

authors： McElligott MC,Menitove JE,Aster RH

更新日期：1986-07-01 00:00:00

abstract：:Sterility of blood products is a cardinal contributor to patient safety. Bacteriologic controls of stable products comply with strict regulations, but legislation imposes only limited constraints in the case of perishable products, such as packed red cells (RBCs) or fresh-frozen plasma (FFP). Therefore, it is essentia...

journal_title：Transfusion

authors： Guéguen M,Durand F,Delamaire D,Beauplet A,Tardivel R,Genetet B,Chalumeau H,Balme MC

更新日期：1986-05-01 00:00:00

abstract：:Fourteen Jk(a-b-) persons were detected by testing 638,460 Osaka blood donors with an automated 2 M urea technique. Two of these 14 Jk(a-b-) samples were quite different from the hitherto reported Jk(a-b-) phenotype, and a family study showed that the mode of the inheritance was dominant. The red cell membranes of the...

journal_title：Transfusion

authors： Okubo Y,Yamaguchi H,Nagao N,Tomita T,Seno T,Tanaka M

更新日期：1986-05-01 00:00:00

abstract：:A unit-dose system was designed to dispense precise quantities of blood in a form ready for immediate transfusion into neonatal patients. The principles were similar to those used by pharmacies to dispense individual doses of drugs. In the blood center, the precise volume of blood ordered for a neonatal patient was as...

journal_title：Transfusion

authors： Strauss RG,Crawford GF,Elbert C,Floss AM,Liesch M

更新日期：1986-03-01 00:00:00

abstract：:A patient with T-polyagglutinable red cells and a severe coagulopathy provided an opportunity to observe the results of plasma transfusion in the face of T-activation. The patient was a 52-year-old Navajo Indian with a perforated gall bladder and related sepsis due to Clostridium perfringens. The gall bladder was remo...

journal_title：Transfusion

authors： Eversole M,Nonemaker B,Zurek K,South S,Simon T

更新日期：1986-03-01 00:00:00

abstract：:Four commercial test kits for detecting cytomegalovirus (CMV) antibodies (indirect hemagglutination assay, indirect fluorescent antibody technique, enzyme immunoassay, and passive latex agglutination technique) were compared according to their technical demand, hands-on time, turnaround time, concordance with other te...

journal_title：Transfusion

authors： Feng CS,Williams IB,Gohd RS,Causey AP

更新日期：1986-03-01 00:00:00

abstract：:We observed complement (C) activation during intermittent flow apheresis procedures (Haemonetics model 30) in four subjects, two of whom developed adult respiratory distress syndrome (ARDS). Actual C3 conversion during apheresis was illustrated by the finding of significantly elevated C3d levels (p less than 0.05) and...

journal_title：Transfusion

authors： Boogaerts MA,Roelant C,Goossens W,Verwilghen RL

更新日期：1986-01-01 00:00:00

abstract：:Currently, platelet concentrates are stored in 50 to 60 ml of plasma. A major drawback to storage in plasma is the considerable loss of platelet function which occurs during storage. A modified Tyrodes medium has been developed for storage of platelets. A comparison between platelet concentrates stored in this medium ...

journal_title：Transfusion

authors： Rock G,Swenson SD,Adams GA

更新日期：1985-11-01 00:00:00

abstract：:We report here the results of our evaluation of two procedures to eliminate viruses in factor VIII and factor IX coagulation factor concentrates. Both procedures were equally effective in the in vitro destruction of marker viruses. However, in a controlled infectivity test in chimpanzees, treatment at 60 degrees C for...

journal_title：Transfusion

authors： Heldebrant CM,Gomperts ED,Kasper CK,McDougal JS,Friedman AE,Hwang DS,Muchmore E,Jordan S,Miller R,Sergis-Davenport E

更新日期：1985-11-01 00:00:00

abstract：:A patient who had a previous anaphylactoid reaction to albumin required plasma exchange for myasthenia gravis. She was rechallenged with albumin from a different manufacturer after premedication with diphenhydramine. No adverse reaction occurred during five exchanges over a 10-day period. Possible pathogenic mechanism...

journal_title：Transfusion

authors： Edelman BB,Straughn MA,Getz P,Schwartz E

更新日期：1985-09-01 00:00:00

abstract：:Exchange transfusions using whole blood less than 24 hours old and deglycerolized red cells reconstituted with fresh-frozen plasma were performed in two premature infants for the treatment of hyperkalemia and cardiac arrhythmia. Improvement of their condition was immediate. ...

journal_title：Transfusion

authors： Lorch V,Jones FS,Hoersten IR

更新日期：1985-07-01 00:00:00

abstract：:Each of 20 donors underwent plateletapheresis on both the CS-3000 (Fenwal) and the 2997 (IBM) blood cell separators. The same operator performed both procedures and conducted all product studies for each donor. Platelet collection efficiency was significantly greater with the 2997 (81%) than the CS-3000 (60%) (p less ...

journal_title：Transfusion

authors： Mintz PD

更新日期：1985-07-01 00:00:00

abstract：:The effects of hydroxyethyl starch (HES) on hemostasis were investigated extensively. In order to simulate acute blood loss due to surgery or trauma, one unit (450 ml) of blood was drawn from normal healthy men. This was followed by a 1-liter infusion over 60 minutes of either 6 percent HES, 5 percent albumin, or 0.9 ...

journal_title：Transfusion

authors： Stump DC,Strauss RG,Henriksen RA,Petersen RE,Saunders R

更新日期：1985-07-01 00:00:00

abstract：:A stable mouse hybridoma line secreting monoclonal anti-C3d antibody (NBTS/BRIC 8) was produced. Ascitic fluid containing BRIC 8 can be used at a final dilution of 1 to 5000 to provide an excellent antiglobulin reagent when mixed with a conventional polyclonal rabbit anti-human IgG serum. ...

journal_title：Transfusion

authors： Holt PD,Donaldson C,Judson PA,Johnson P,Parsons SF,Anstee DJ

更新日期：1985-05-01 00:00:00

abstract：:The whole blood electrical conductivity method for predonation hematocrit determinations was studied. Forty capillary and venous blood samples were tested concurrently with electrical conductivity and centrifugation methods. The electrical conductivity method demonstrated acceptable accuracy but was less precise than ...

journal_title：Transfusion

authors： Schifman RB,Rivers SL,Delduca M

更新日期：1985-05-01 00:00:00

abstract：:Clinical and serological data on 1435 Italian thalassemia major patients were collected during a cooperative study involving 19 centers in 10 regions. The main findings were as follows: 18 percent of the patients were under 6 years of age, 63 percent between 6 and 15, and 19 percent over 15. Forty-one percent had unde...

journal_title：Transfusion

authors： Sirchia G,Zanella A,Parravicini A,Morelati F,Rebulla P,Masera G

更新日期：1985-03-01 00:00:00

abstract：:We investigated the possibility that the functional impairment in neutrophil (PMN) chemotaxis which occurs during granulocyte concentrate storage might be due to autotoxicity from the release of neutrophil granule contents during storage. Preliminary experiments confirmed that the exposure of fresh PMNs to the intrace...

journal_title：Transfusion

authors： Lane TA,Lamkin GE

更新日期：1985-03-01 00:00:00

abstract：:A solid-phase adherence method (SPAM) for ABO grouping and Rh typing of red cells (RBCs) has been developed. Adherence reactions were read spectrophotometrically and interpreted by a computer. The SPAM had a 99.6 percent correlation with conventional microplate agglutination methods for ABO grouping and Rh typing. The...

journal_title：Transfusion

authors： Sinor LT,Rachel JM,Beck ML,Bayer WL,Coenen WM,Plapp FV

更新日期：1985-01-01 00:00:00

abstract：:A 27-year-old, gravida 3, para 2 woman experienced a delayed hemolytic transfusion reaction. She had anti-Dob in both serum and eluate 8 days after infusion of 6 units of Do(b+) red cells. No antibody had been detected prior to transfusion. By the 15th day after transfusion, there was no evidence of survival of red ce...

journal_title：Transfusion

authors： Moheng MC,McCarthy P,Pierce SR

更新日期：1985-01-01 00:00:00

abstract：:The development of flexible plastic blood bags has permitted effective blood component production and therapy. However, the plasticizer di(2-ethylhexyl)phthalate (DEHP), whose toxicity in humans is still undefined, is known to leach from the plastic into stored blood. Despite the availability of bags made of plastics ...

journal_title：Transfusion

authors： Rock G,Tocchi M,Ganz PR,Tackaberry ES

更新日期：1984-11-01 00:00:00

abstract：:Cryoprecipitate is used infrequently in home therapy for patients with hemophilia A since freezer storage is required and resuspension and pooling of thawed cryoprecipitate is cumbersome. We evaluated procedures for preparation of cryoprecipitate in an "open system" so that four to six bags of cryoprecipitate could be...

journal_title：Transfusion

authors： Smith KJ,Hodges PA

更新日期：1984-11-01 00:00:00

abstract：:This study was designed to test the viability of two multiple request techniques of behavioral influence for recruiting blood donors by telephone. The first technique utilizes a small antecedent request to encourage behavioral involvement and favorable disposition toward the target activity of the critical request to ...

journal_title：Transfusion

authors： Hayes TJ,Dwyer FR,Greenwalt TJ,Coe NA

更新日期：1984-09-01 00:00:00